Coding

Part:BBa_K3944034

Designed by: Olof Dahlman   Group: iGEM21_Chalmers-Gothenburg   (2021-09-30)


zif268-ER LDB-VP16

Fusion protein consisting of protein zif268 which binds to repeats of the Z3BS recognition sequence, estradiol-recognizing domain ER LDB which triggers conformational change and activity upon binding to estradiol, and viral transcription protein VP16, transcribing genes downstream fusion protein upon binding to DNA sequence. The whole complex will trigger transcription of genes downstream of the Z3BS repeats upon addition of the induction agent estradiol in a dose-dependent manner (1).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 166
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None